20X TBE - Nucleic Acid Electrophoresis Applications
20X TBE is a high‑ionic‑strength stock (diluted typically to 1X or 0.5X) providing stable pH and conductivity for high‑resolution separation of nucleic acids.It contains Tris base, borate, and EDTA to maintain pH around 8.1-8.3 and chelate divalent cations, protecting DNA/RNA from nuclease degradation during runs.Molecular biology labs use it for casting agarose gels, running tanks, and re‑running fragments where sharp bands and minimal smiling are required.Sterile, filtered formulations with long shelf life support reproducible electrophoresis performance across multiple gel batches.
